A human hepatic 3 D cell model for the mechanistic investigation of N-acetyl-p-aminophenol (APAP)
Sprache des Vortragstitels:
IVTIP Spring 2011 Meeting
Sprache des Tagungstitel:
The human hepatoma cell line HepaRG, able to differentiate into hepatocyte-like cells, was cultured in a 2D or a 3D model. Cells were seeded on porous plasma activated Polystyrene (PS) scaffolds to supply more physiological culture conditions (3D) or in 24-well PS culture plates (2D). HepaRG cells were seeded at 1x106 cells per polystyrene
sponge and cultured for one day or one week. To differentiate, cells were
treated with 1% DMSO for 1 day or 28 days.