Sensing single molecules with the Atomic Force Microscope: New approaches in tip chemistry
Sprache des Vortragstitels:
Englisch
Original Tagungtitel:
SMCBS 2013
Sprache des Tagungstitel:
Englisch
Original Kurzfassung:
Atomic Force Microscopy (AFM) has developed to an important tool in life sciences. It allows determining the surface roughness of soft biological samples like cells and membranes at near physiological conditions, label-free, and with a lateral resolution in the nanometer range. In addition, upgrading the usually inert AFM tip by tethering biological molecules to the tip allows gaining more information. Topography and Recognition Imaging[1] combines tapping mode imaging with a biosensing AFM tip[2] and allows to simultaneously map the surface topography as well as to localize specific binding partners[3]. A second technique, also based on the use of molecular sensing bio-functionalized AFM tips, is Molecular Recognition Force Spectroscopy[4]. There a biosensing tip is repeatedly approached and withdrawn from a surface containing the corresponding binding partner. Within every force distance cycle a ligand-receptor complex can be formed and ruptured. By varying the pulling velocities the complex energy landscape can be explored giving insights into kinetic and energetic aspects of the interaction [5]. Common and new approaches in the generation of single molecule sensing AFM tips for TREC and MRFS as well as recent applications will be shown.
Sprache der Kurzfassung:
Englisch
Englischer Vortragstitel:
Sensing single molecules with the Atomic Force Microscope: New approaches in tip chemistry
Vortragstyp:
Hauptvortrag / Eingeladener Vortrag auf einer Tagung
Forschungs-