Multiplex Quadrature Detection in Multidimensional NMR
Sprache des Vortragstitels:
Austrian Chemistry Days 2011
Sprache des Tagungstitel:
Application of multiplex phase cycling  to multi-dimensional heteronuclear NMR experiments significantly reduces the number of acquisitions required to achieve the desired resolution in the indirect dimensions.
In conventional N-dimensional pulse sequences the minimum number of one- dimensional FIDs required to obtain a phase-sensitive spectrum is determined by the basic phase cycle and the method for quadrature detection (e.g. States, TPPI, echo-/ anti-echo).
With multiplex quadrature detection (MQD) the tasks of coherence selection and quadrature separation are merged. The minimum total number of scans, which have to be combined per data point, is decreased by a factor (3/4)N-1. This is especially interesting for three- and higher dimensional experiments since spectra with the same resolution in the indirect dimension can be obtained in less time. Furthermore this approach can straightforwardly be combined with other time saving methods such as sparse sampling techniques [2,3,4,5].
We present MQD 3D HNCO spectra of the photosynthetic protein PsbQ . We could obtain the same resolution and the same per scan signal to noise ratio as in the standard HNCO in only 56% of the usual time.