Sodikdjon Kodirov, Tobias Herbinger, Arndt Rohwedder,
"Comparable properties of native K channels in the atrium and ventricle of snails"
, in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, Vol. 282, Elsevier, 5-2024
Original Titel:
Comparable properties of native K channels in the atrium and ventricle of snails
Sprache des Titels:
Englisch
Original Kurzfassung:
Mollusks, including snails, possess two chambered hearts. The heart and cardiomyocytes of snails have many
similarities with those of mammals. Also, the biophysics and pharmacology of Ca, K, and Na ion channels
resemble. Similar to mammals, in mollusks, the ventricular cardiomyocytes and K channels are often studied,
which are selectively sensitive to antagonists such as 4-AP, E-4031, and TEA. Since the physiological properties
of the ventricular cardiac cells of snails are well characterized, the enzymatically dissociated atrial car
diomyocytes of Cornu aspersum (Müller, 1774) were studied using the whole-cell patch-clamp technique for
detailed comparisons with mice, Mus musculus. The incubation of tissues in a solution simultaneously containing
two enzymes, collagenase and papain, enabled the isolation of single cells. Recordings in the atrial car
diomyocytes of snails revealed outward K+currents closely resembling those of the ventricle. The latter was
consistent, whether the voltage ramp or steps and long or short pulses were used. Interestingly, under identical
conditions, the current waveforms of atrial cardiomyocytes in snails were similar to those of mice left ventricles,
albeit the kinetics and the absence of inward rectifier K channel (IK1) activation. Therefore, the heart of mollusks
could be used as a simple and accessible experimental model, particularly for pharmacology and toxicology
studies.
Sprache der Kurzfassung:
Englisch
Journal:
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Forschungs-