Axon onset remodeling in response to network activity in mouse cortical neurons
Sprache des Titels:
Englisch
Original Kurzfassung:
In certain subtypes of pyramidal neurons, axons originate from basal dendrites, resulting in an axon-carrying dendrite branch with unique functional characteristics (AcD cells). The number of AcD cells increases during development, however, it is so far unclear whether neurons remodel their axon emergence throughout their lifetime in response to changes in network activity. To test our hypothesis of such large-scale morphological plasticity, we utilized in vitro and in vivo strategies in mouse primary somatosensory cortex to test whether network activity impacts axon onset. Based on data obtained by immunofluorescence, confocal microscopy and/or live-cell imaging, we show that neurons are indeed capable of changing the onset of their axon origin from somatic to dendritic and vice versa within a few days in vitro and that peripheral whisker manipulation and thus changes in sensory input drives large-scale morphological plasticity in vivo.
Forschungs-