Aqueous angiography (AA) is a new imaging technique that allows real-time, physiological visualization of aqueous outflow pathways beyond Schlemm's canal (SC). Recent studies with AA have shown the segmental nature of these outflow pathways, which can significantly alter our understanding of the success and failure of minimally invasive glaucoma surgeries (MIGS) and help us in customizing as well as devising new treatment strategies which can reduce intraocular pressure (IOP) with minimal tissue damage. The main limitation of AA is that it is an invasive procedure which limits its use in the follow-up period after surgery.