Jan Kern, Frank Müh, A. Zouni,
"Structural Studies on tetrapyrrole containing proteins enabled by femtosecond X-ray pulses"
, in Bernhard Grimm: Metabolism, Structure and Function of Plant Tetrapyrroles: Control Mechanisms of Chlorophyll Biosynthesis and Analysis of Chlorophyll-Binding Proteins, Serie Advances in Botanical Research, Vol. 91, 2019, ISBN: 978-0-12-819982-4
Original Titel:
Structural Studies on tetrapyrrole containing proteins enabled by femtosecond X-ray pulses
Sprache des Titels:
Englisch
Original Buchtitel:
Metabolism, Structure and Function of Plant Tetrapyrroles: Control Mechanisms of Chlorophyll Biosynthesis and Analysis of Chlorophyll-Binding Proteins
Original Kurzfassung:
The development of the X-ray free electron laser (XFEL) as a tool for crystallography started a new era in protein science with the intriguing possibility of determining time-resolved structural changes under physiological conditions. This chapter summarizes the current state of XFEL-based structural research on tetrapyrrole containing proteins including the photosystems of oxygenic photosynthesis, the purple bacterial reaction center, phycobilins, and phytochromes. Besides describing technical developments that were necessary to fully exploit the advantages of the method, emphasis is put on the task of producing suitable protein microcrystals for XFEL experiments. In particular, issues encountered in crystallizing membrane proteins are described with photosystem II as the prime example to underscore the importance of combining physical and biochemical knowledge, but also to point to unsolved problems. Finally, novel structural information obtained by XFEL experiments for the various systems is given with a focus on recent advances in understanding photosynthetic water oxidation.
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