Reply to Comment on "Elastic Membrane Deformations Govern Interleaflet Coupling of Lipid-Ordered Domains"
Sprache des Titels:
Englisch
Original Kurzfassung:
Our recent publication in this journal [1] challenges the concept that domains in opposing membrane leaflets are in register because of interactions at a membrane midplane. Compelled by the lack of direct experimental proof for (i) midplane interaction via an overhang [2] or (ii) LO and LD phases repelling each other [3] we propose that minimization of line tension ? drives registration (R) [1]. We dismiss antiregistration (AR) as an unlikely event because its twofold larger domain area translates into a 2?-fold larger boundary length. Moreover, the line tensions at the LD/LD?LD/LO and LO/LO?LD/LO interfaces (Cartoon 1), ?DD and ?OO, respectively, exceed the line tension at the LD/LD?LO/LO interface, ?R rendering the elastic energy WR of the registered state smaller than the elastic energy WAR of the antiregistered state. Consequently, registration is energetically favorable. Also, ?R > ?DD, ?OO because an isolated LD/LO boundary in only one leaflet leads to membrane bending. As readily observed in the Cartoon, for the membrane to remain flat, a substantial torque must be applied or an LD/LO boundary must be created in the upper monolayer to oppose the LD/LO boundary in the lower monolayer.
Forschungs-