VWF - Collagen Interactions Studied with Single Molecule Force Spectroscopy
Sprache des Titels:
Englisch
Original Kurzfassung:
Von Willebrand factor (VWF) is a huge multimeric protein that plays a key role in hemostasis. Sites for collagen binding, an initial event of hemostasis, are located in domains A1 and A3 of VWF. Collagen III is believed to interact with the A3-domain, and collagen VI with the A1-domain. The forces and the dynamics of these interactions were investigated with molecular recognition force spectroscopy (MRFS), using substrates with a dense layer of poly (ethylene glycol) chains and terminal benzaldehyde functions for covalent immobilization of collagen.Von Willebrand factor (VWF) is a huge multimeric protein that plays a key role in hemostasis. Sites for collagen binding, an initial event of hemostasis, are located in domains A1 and A3 of VWF. Collagen III is believed to interact with the A3-domain, and collagen VI with the A1-domain. The forces and the dynamics of these interactions were investigated with molecular recognition force spectroscopy (MRFS), using substrates with a dense layer of poly (ethylene glycol) chains and terminal benzaldehyde functions for covalent immobilization of collagen.
Forschungs-