Wolfgang Schöfberger,
"Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803"
, in Photosynthetica, Vol. 43, Nummer 3, 2005, ISSN: 1573-9058
Original Titel:
Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803
Sprache des Titels:
Englisch
Original Kurzfassung:
The PsbH protein of cyanobacterium Synechocystis sp. PCC 6803 was expressed as a fusion protein with glutathione-S
transferase (GST) in E. coli grown on a mineral medium enriched in 15N isotope. After enzymatic cleavage of the fusion
protein, the 1H-15N-HSQC spectrum of PsbH protein in presence of the detergent β-D-octyl-glucopyranoside (OG) was
recorded on a Bruker DRX 500 MHz NMR spectrometer equipped with a 5 mm TXI cryoprobe to enhance the sensitivity
and resolution. Non-labelled protein was used for secondary structure estimation by de-convolution from circular dichroism
(CD) spectra. Experimental results were compared with our results from a structural model of PsbH using a restraint-
based comparative modelling approach combined with molecular dynamics and energetic modelling. We found
that PsbH shows 34–38 % α-helical structure (Thr36-Ser60), a maximum of around 15 % of β-sheet, and 12–19 % of
β-turn.
Forschungs-