Sepp Hochreiter, Klaus Obermayer, Djork-Arné Clevert,
"A new summarization method for affymetrix probe level data"
, in Bioinformatics, Vol. 22, Nummer 8, Seite(n) 943-949, 2006, ISSN: 1367-4811
Original Titel:
A new summarization method for affymetrix probe level data
Sprache des Titels:
Englisch
Original Kurzfassung:
We propose a new model-based technique for summarizing high-density oligonucleotide array data at probe level for Affymetrix GeneChips. The new summarization method is based on a factor analysis model for which a Bayesian maximum a posteriori method optimizes the model parameters under the assumption of Gaussian measurement noise. Thereafter, the RNA concentration is estimated from the model. In contrast to previous methods our new method called ‘Factor Analysis for Robust Microarray Summarization (FARMS)’ supplies both P-values indicating interesting information and signal intensity values.