Osteoarthritis (OA) is one of the leading musculoskeletal disorders in the aging population and still a challenge for clinicians and scientists. Currently, there is no long-lasting regenerative treatment for OA available. Tissue engineering has emerged as a promising tool for osteochondral (OC) defect restoration. In this project, we want to interrupt inflammation and matrix degradation present in OA while reconstructing new tissue using innovative allogeneic stem cells with strong anti-inflammatory and immunomodulatory properties and high differentiation potential. To develop and manufacture a therapeutic stem cell product, extensive quality control is required to ensure the identity, purity, potency and safety of the cells by avoiding genetic instabilities and tumorigenicity. Our aim is to establish a safe and efficient basis for truly allogeneic cell therapies by defining preliminary Quality Target Product Profile (QTPP)s.