Torben Hoomann, Nadine Jahnke, Andreas Horner, Sandro Keller, Peter Pohl,
"Filter gate closure inhibits ion but not water transport through potassium channels"
, in Proceedings of the National Academy of Sciences of the United States of America (PNAS), Vol. 110, Nummer 26, Seite(n) 10842-10847, 6-2013, ISSN: 1091-6490
Original Titel:
Filter gate closure inhibits ion but not water transport through potassium channels
Sprache des Titels:
Englisch
Original Kurzfassung:
The selectivity filter of K+ channels is conserved throughout all kingdoms of life. Carbonyl groups of highly conserved amino acids point toward the lumen to act as surrogates for the water molecules of K+ hydration. Ion conductivity is abrogated if some of these carbonyl groups flip out of the lumen, which happens (i) in the process of C-type inactivation or (ii) during filter collapse in the absence of K+. Here, we show that K+ channels remain permeable to water, even after entering such an electrically silent conformation. We reconstituted fluorescently labeled and constitutively open mutants of the bacterial K+ channel KcsA into lipid vesicles that were either C-type inactivating or noninactivating. Fluorescence correlation spectroscopy allowed us to count both the number of proteoliposomes and the number of protein-containing micelles after solubilization, providing the number of reconstituted channels per proteoliposome. Quantification of the per-channel increment in proteoliposome water permeability with the aid of stopped-flow experiments yielded a unitary water permeability pf of (6.9 ± 0.6) × 10?13 cm3?s?1 for both mutants. ?Collapse? of the selectivity filter upon K+ removal did not alter pf and was fully reversible, as demonstrated by current measurements through planar bilayers in a K+-containing medium to which K+-free proteoliposomes were fused. Water flow through KcsA is halved by 200 mM K+ in the aqueous solution, which indicates an effective K+ dissociation constant in that range for a singly occupied channel. This questions the widely accepted hypothesis that multiple K+ ions in the selectivity filter act to mutually destabilize binding.
Sprache der Kurzfassung:
Englisch
Journal:
Proceedings of the National Academy of Sciences of the United States of America (PNAS)